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Recombinant Human B7-2/CD86, Fc Chimera (HEK293-expressed)
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| 貨號 | 規格 | 貨期 | 庫存 | 價格 | 促銷價 | 訂購 |
| 1308132833-10 μg | 0 | ￥2200 | ||||
| 1308132833-50 μg | 0 | ￥4200 |
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| 性狀 | Recombinant Human B7-2/CD86, Fc Chimera (HEK293-expressed) 是在 HEK293 細胞中產生的 C-末端標記 IgG1 Fc 片段的多肽鏈。B7-2 (CD86) 是由抗原呈遞細胞 (APC) 表達的共刺激分子，與 CD28 相互作用來促進 T 細胞活化和存活，并與 CTLA4 相互作用來進行免疫調節。
Synonyms rHuB7-2/CD86, Fc Chimera; Activation B7-2 antigen; B70; BU63; CD86; CD28LG2 Species Human Source HEK 293 Accession P42081 Gene ID 942 Molecular Weight 65-80 kDa AA Sequence LSGAAPLKIQ AYFNETADLP CQFANSQNQS LSELVVFWQD QENLVLNEVY LGKEKFDSVH SKYMGRTSFD SDSWTLRLHN LQIKDKGLYQ CIIHHKKPTG MIRIHQMNSE LSVLANFSQP EIVPISNITE NVYINLTCSS IHGYPEPKKM SVLLRTKNST IEYDGVMQKS QDNVTELYDV SISLSVSFPD VTSNMTIFCI LETDKTRLLS SPFSIELEDP QPPPDHIP Biological Activity 5 μg/mL (100 μL/well) of immoblized recombinant human B7-2/CD86-Fc can bind human Biotin-CD28-Fc with a linear range of 0.09-3.12 μg/mL. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol. Endotoxin Level <0.2 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Human B7-2/CD86, Fc Chimera (HEK293-expressed) (rHuB7-2/CD86, Fc Chimera) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability Lyophilized recombinant Human B7-2/CD86, Fc Chimera (HEK293-expressed) (rHuB7-2/CD86, Fc Chimera) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background CD86 is a transmembrane region, and a longer cytoplasmic domain than CD80. CD86 is constitutively expressed on interdigitating dendritic cells (DCs), Langerhans cells, peripheral blood DCs, memory B cells and germinal center B cells, and macrophages. CD86 is rapidly upregulated on B cells following activation by cross-linking of the Ig receptor or the addition of a variety of cytokines[1]. |
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